AUCTORES
Globalize your Research
Review Article | DOI: https://doi.org/10.31579/2690-8816/163
1Professor, Department of Pharmaceutics, Sri Ramakrishna Institute of Paramedical Sciences, College of Pharmacy, Tamil Nadu, Coimbatore – 44, India.
2B. Pharm, Sri Ramakrishna Institute of Paramedical Sciences, College of Pharmacy, Tamil Nadu, Coimbatore – 44, India.
*Corresponding Author: J Bagya lakshmi. Professor, Department of Pharmaceutics, Sri Ramakrishna Institute of Paramedical Sciences, College of Pharmacy, Tamil Nadu, Coimbatore – 44, India.
Citation: J Bagya lakshmi, K Vibisha, M Vignesh, N Vijay, R Vijayalakshmi, et al, (2025), Green Synthesized Silver Nanoparticles of Artocarpus Heterophyllus Peel Extract and Its Evaluation for Anti-Inflammatory Activity, J Clinical Research Notes, 6(2); DOI:10.31579/2690-8816/163
Copyright: © 2025, J Bagya Lakshmi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 18 March 2025 | Accepted: 26 March 2025 | Published: 01 April 2025
Keywords: artocarpus heterophyllus; silver nanoparticles;green synthesis; anti-inflammatory
The aim of the study was to formulate Artocarpus heterophyllus peel extract into silver nanoparticles using green synthesis method and evaluating its Anti-inflammatory activity. It has various pharmacological benefits like anti-inflammatory, carcinogenic, anti-tubercular, anti-diabetic, anti-infective, anti-fungal, anti-viral, anti-oxidant. Artocarpus heterophyllus peel was extracted using methanol by maceration method and synthesized into silver nanoparticles using 1Mm silver nitrate by green synthesis. Then the nanoparticle was evaluated using particle size, zeta potential, UV-visible, and visual examination. The in vitro anti-inflammatory protein denaturation method using egg albumin in green synthesized Artocarpus heterophyllus peel extract silver nanoparticle was eco-friendly, non- toxic when compared to synthetic anti- inflammatory drugs.
Silver Nanoparticles
The investigation of the therapeutic properties of AgNPs has allowed studies to be carried out on the mechanism for modulating cytokines. This research could allow the development of new treatments, define the effective therapeutic concentrations and indications for use as well as evaluate the cytotoxic, genotoxic and carcinogenic potential and associated environmental impact. AgNPs show a reduction in pro-inflammatory proteins and in the selective inhibition of the COX-2 pathway. It was concluded that AgNPs presents an anti-inflammatory action in vivo through mechanisms involving the increase of anti -inflammatory molecules.
Green Synthesis
Green synthesis is an emerging area in the field of science and provides both economic and environmental benefits. In this study, non- toxic reagents that are eco- friendly and biosafe are used. The use of herbal extracts in the green synthesis of metal oxide nanoparticles is an increasing approach.
Inflammation
Inflammation that can be triggered by a variety of factors including pathogens, damaged cells and toxic compounds. These factors may induce acute and chronic inflammatory responses in the heart, pancreas, lung, brain, intestinal tract and reproductive system potentially leading to tissue damage or disease. Both infectious and non -infectious agents and cell damage activate inflammatory cells and trigger inflammatory signaling pathways, most commonly the NK-B, MAPK and JAK STAT pathways. These signals activate LEUKOCYTE CHEMOTAXIS from the general circulation to sites of damage. These activated leukocytes produce cytokines that induce inflammatory responses.
Anti Inflammatory Activity
Anti-inflammatory drugs that inhibit H1 Histamine, COX enzyme and reduce the synthesis of prostanoids. Corticosteroids prevent the formation of both PGs and LTs. The inhibition of phospholipase A2 reduces arachidonic acid release and suppresses the inflammation.
Materials
Artocarpus heterophyllus peel powder
Chemicals Reagents
Silver nitrate (AgNO3), distilled water.
Equipment Required
Magnetic stirrer, UV-Visible spectrophotometer, Homogenizer, Malvern Zeta size analyzer
Procedure:
Sample Collection
Artocarpus heterophyllus peels were collected from Coimbatore district.
Authentication
The plant specimen was identified and authenticated from Tamil Nadu Agriculture university.
Drying and Pulverizing
The peels were collected and shade dried. It was chopped into small pieces and kept for extraction.
Preparation of extract of peel of Artocarpus heterophyllus
Artocarpus heterophyllus fruits in total were collected, and each fruit's surface was cleaned with water and allowed to air dry. After cutting each fruit, the peel and pulp were separated. Carefully, the rinds, skin, and spines were separated. All of the fruits' spine, and skin samples were taken, chopped into tiny bits, and then blended. Each smashed piece was weighed separately. For 72 hours, 300 g of each were soaked in a 1:5 ratio (100 gm in 500 ml) of seven distinct solvents (polar-ethanol, methanol, ethyl acetate, non-polar chloroform, n-hexane, dimethyl sulfoxide, and petroleum ether) at room temperature. Filtration was used to separate the supernatant fluid, and a hot water bath was used to dry the residue. It was taken for analysis since the ethanolic extraction yield from all three sections was determined to be higher.
Synthesis of Silver Nanoparticles
In a typical reaction procedure, the 1 mM (mM) of AgNO3 aqueous solution was prepared in 100 ml Distilled water in 250 ml beaker and was used to produce silver Nanoparticles. The 40 ml of 1 mM AgNO3 solution was taken and treated with 10 ml of peel extract filtrate and heated the mixture for 1 hour at 40-50°C for reduction into Ag ions. For 1 mM silver nitrate solution the leaf extract acts as a reducing and stabilizing agent. The resulting solution becomes grey-black in colour after 48 hours, indicating the formation of nanoparticles. The concentrations of peel extract were also varied from 4 ml to 10 ml leaves extract as well as temperature and time duration are also varied.
Preformulation Studies
Solubility
Solubility test of Artocarpus heterophyllus peel were tested using different solvents such as ethanol, methanol, water and the results were given below.
| SOLVENTS | SOLUBILITY |
| Water | Insoluble |
| Ethanol | Completely soluble |
| Methanol | Completely soluble |
Table 1: Solubility of Artocarpus heterophyllus peel extract
Characterization of Synthesized Artocarpus heterophyllus Silver Nanoparticle
Characterization of Artocarpus heterophyllus silver nanoparticles is done to evaluate the functional properties. Various analytical techniques are utilized to confirm their formation and evaluate their characteristics. These techniques include UV-Visible spectroscopy, particle size analysis and zeta potential measurement.
Visual Examination
The primary confirmation of the synthesized Artocarpus heterophyllus silver nanoparticles is done by visual basis. The colour change of reaction mixture (silver nitrate solution and peel extract) with respect to time is observed.
UV-VIS spectroscopy
UV-VIS spectroscopy is a valuable technique to characterize the transmission, absorption and reflectivity of several compounds and for scientifically important material, such as coating and pigments etc. The ultraviolet and visible spectrum have wide features that are of use for sample identification but are extremely helpful for quantitative measurements.
Determination of particle size
The dried powders of Artocarpus heterophyllus Silver Nanoparticles dispersed in water to obtain proper scattering intensity of Artocarpus heterophyllus silver nanoparticles. The particle size was determined by the Malvern zeta size analyser.
Determination of zeta potential
The zeta potential was measured by using Zeta Sizer (Malvern Instruments) having zeta cells,
polycarbonate cell with gold-plated electrodes and using water as medium for sample preparation. Zeta potential determines the surface potential of silver nanoparticles and it is essential for the characterization of stability of nanoparticles.
In-Vitro Methods
Anti-arthritic studies by protein denaturation method
In Vitro anti-arthritic assay was conducted by protein denaturation method given by Mizushima and Kobayashi (1968). The reaction mixture (5 ml) consisted of 0.2 ml of egg albumin, 2.8 ml of phosphate-buffered saline (PBS with pH 6.4), and 2 ml of varying concentrations (10, 20,50, 10 ug/L) of plant extract. A similar volume of double distilled water is used as control. Then the mixture was incubated at 37 °C in a BOD incubator for 15 min and then heated at 70 °C for 5 min. After cooling, their absorbance was measured at 660 m by using the vehicle as blank.
The percentage of inhibition of protein denaturation was calculated by using the following formula;
Inhibition = AC660-AT660 x 100 AC600
AC = Absorbance of control solution, AT = Absorbance of the test sample.
Egg Albumin Assay
The anti-inflammatory activity of unknown crude extracts can be determined in vitro for inhibition of the denaturation of egg albumin (protein).
* 0.2 mL of 1-2% egg albumin solution (from fresh hen's egg or commercially available egg albumin powder), 2 mL of sample extract or standard (methotrexate) at varying concentrations, and 2.8 mL of phosphate buffer saline (pH 7.4) were mixed to form a reaction mixture of a total volume of 5 ml.
* A total volume of 5 mL of the control was created by combining 2 mL of triple-distilled water, 0.2 mL of 1-2% egg albumin solution, and 2.8 mL of phosphate-buffered saline.
* The reaction mixtures were then incubated at 37±2°C for 30 min and will be heated in a water bath at 70±2°C for 15 min.
* After cooling, the absorbance was measured at 280 nm by a suitable UV/Vis spectrophotometer using triple distilled water as the blank.
* The following equation was used to determine the percentage inhibition of protein denaturation:
Percentage Inhibition = (Absorbance of control - Absorbance of test sample) / Absorbance of control * 100
* Then plant extract/positive control concentration for 50% inhibition (IC50) was determined by plotting percentage inhibition concerning control against concentration. All determinations were carried out in triplicate.
Characterization of green synthesized artocarpus heterophyllus silver nanoparticles
Visual Examination
The colour of Artocarpus heterophyllus extract in the initial stage was yellow, after the addition of silver nitrate solution it turned into brown colour. There is no colour change after 90minutes, indicating that the reaction is finished. After 48 hrs the colour change from yellow to brown indicates the formation of silver nanoparticles. The image of Artocarpus heterophyllus extract is depicted in figure 6: The image of Artocarpus heterophyllus extract is depicted in figure 7: which denotes the formation of silver nanoparticles after 48hrs.
UV VISIBLE Spectral Analysis OF Artocarpus heterophyllus Silver Nanoparticles
Silver nanoparticles production and completion are characterized by UV-Visible spectral analysis, which makes use of a UV-Visible spectrophotometer.
The production of silver ions to silver nanoparticles was indicated by colour change from yellow to brown colour and it was reflected in spectral data obtained by using UV-Visible spectrophotometer. The silver nanoparticles Surface Plasmon Resonance (SPR) is characterized by the coloration. It shows an absorption peak at 425nm, confirming the formations of Artocarpus heterophyllus silver nanoparticles.
Particle size measurement:
The particle size is one of the most important parameters for Characterization nanoparticles. The mean particle size(z -average), polydispersity index (PI) of Artocarpus heterophyllus silver nanoparticles were determined by using a zeta size analyser (Malvern Instrument). The average particle size of Artocarpus heterophyllus was found to be 60.45nm. Particle size analysis showed the presence of nanoparticles with polydispersity indices PDI value 0.356 with intercept 0.845.
Zeta Potential
Zeta potential was used to determine the stability of prepared nanoparticles. Zeta potential was determined using a zeta-sizer instrument. The surface charge of the particles and stability of the solution was characterized by zeta potential. For Artocarpus heterophyllus silver nanoparticles, zeta potential was found to be 0.304mV with peak area 46.9 intensity. These values indicate that Artocarpus heterophyllus silver nanoparticles are stable.
Invitro Anti- Inflammatory Activity
Anti- inflammatory activity of standard drug aspirin and the Artocarpus heterophyllus silver nanoparticles were determined using protein denaturation assay. The anti- inflammatory activity of unknown crude extracts can be determined by using in vitro for inhibition of the denaturation of egg albumin. Different concentrations of drug sample and egg albumin solution are taken and mixed with phosphate buffer on PH 7.4 and incubated for 30min. Reaction mixture was heated at the water bath for 70°C for 15minutes. After cooling, the absorbance was measured at 280 nm by a suitable UV/Vis spectrophotometer using triple distilled water as the blank. Aspirin is used as standard and the drug sample was compared with standard.
From the above standard drug is compared with our plant extract and the inhibition concentration value for inflammatory prepared nanoparticles of Artocarpus heterophyllus was slightly higher when compared to the standard drug aspirin.
It is believed that protein denaturation is the factor that promotes inflammation. Inhibition activity of Artocarpus heterophyllus silver nanoparticles against Rheumatoid were observed, hence it can be used as a potential alternative to synthetic drugs as a non-toxic safe way of green synthesis.
Figure 1: UV Visible spectrum of Formulation of Artocarpus heterophyllus Nanoparticles
Figure 2: Determination of Particle Size of Artocarpus heterophyllus Silver Nanoparticle
Figure 3: Determination of Zeta Potential of Artocarpus heterophyllus silver nanoparticles
Concentration (µg/ml) | Absorbance | % Inhibition | Average | ||||
| T1 | T2 | T3 | T1 | T2 | T3 | ||
| 10 | 0.0665 | 0.0652 | 0.0646 | 67.08 | 63.81 | 62.31 | 64.40 |
| 20 | 0.0618 | 0.0630 | 0.0627 | 58.27 | 58.29 | 57.03 | 57.03 |
| 30 | 0.0491 | 0.0496 | 0.0506 | 23.37 | 24.62 | 27.14 | 25.04 |
| 40 | 0.0659 | 0.0672 | 0.0661 | 65.57 | 68.84 | 66.08 | 66.82 |
| 50 | 0.0558 | 0.0573 | 0.0580 | 40.20 | 43.97 | 45.73 | 43.30 |
Table 2: Protein Inhibitory Data of Control Aspirin
Concentration (µg/ml) | Absorbance | % Inhibition | Average | ||||
| T1 | T2 | T3 | T1 | T2 | T3 | ||
| 10 | 0.0445 | 0.0450 | 0.0456 | 11.30 | 13.06 | 14.57 | 13.14 |
| 20 | 0.0623 | 0.0633 | 0.0634 | 56.53 | 59.05 | 59.55 | 58.37 |
| 30 | 0.0712 | 0.0725 | 0.0732 | 78.89 | 82.16 | 83.91 | 81.65 |
| 40 | 0.0841 | 0.0832 | 0.0830 | 111.30 | 109.04 | 108.54 | 109.62 |
| 50 | 0.1092 | 0.1097 | 0.1101 | 174.37 | 175.62 | 176.63 | 175.54 |
Table 3: Protein Inhibitory Data of Artocarpus heterophyllus Silver Nanoparticles
The study shows green synthesized silver nanoparticles of Artocarpus heterophyllyus has anti-inflammatory activity. These nanoparticles were characterized and evaluated by various test such as visual examination, UV-VIS Spectral analysis, zeta potential. Invitro anti-inflammatory activity was studied. Perform invivo methods for further confirmation of above mentioned property.
Clearly Auctoresonline and particularly Psychology and Mental Health Care Journal is dedicated to improving health care services for individuals and populations. The editorial boards' ability to efficiently recognize and share the global importance of health literacy with a variety of stakeholders. Auctoresonline publishing platform can be used to facilitate of optimal client-based services and should be added to health care professionals' repertoire of evidence-based health care resources.
Journal of Clinical Cardiology and Cardiovascular Intervention The submission and review process was adequate. However I think that the publication total value should have been enlightened in early fases. Thank you for all.
Journal of Women Health Care and Issues By the present mail, I want to say thank to you and tour colleagues for facilitating my published article. Specially thank you for the peer review process, support from the editorial office. I appreciate positively the quality of your journal.
Journal of Clinical Research and Reports I would be very delighted to submit my testimonial regarding the reviewer board and the editorial office. The reviewer board were accurate and helpful regarding any modifications for my manuscript. And the editorial office were very helpful and supportive in contacting and monitoring with any update and offering help. It was my pleasure to contribute with your promising Journal and I am looking forward for more collaboration.
We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.
The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.
Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.
Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.
Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.
Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.
Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.
This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.
Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.
As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.
Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.
International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.
Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.
Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.
I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!
"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".
I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.
We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.
I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.
I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.
I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.
Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.
“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.
Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.
My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.
My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.
I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.
Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."
I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.
To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.
"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".
I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.
Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.
We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.
My article, titled 'No Way Out of the Smartphone Epidemic Without Considering the Insights of Brain Research,' has been republished in the International Journal of Clinical Case Reports and Reviews. The review process was seamless and professional, with the editors being both friendly and supportive. I am deeply grateful for their efforts.
To Dear Erin Aust – Editorial Coordinator of Journal of General Medicine and Clinical Practice! I declare that I am absolutely satisfied with your work carried out with great competence in following the manuscript during the various stages from its receipt, during the revision process to the final acceptance for publication. Thank Prof. Elvira Farina
Dear Jessica, and the super professional team of the ‘Clinical Cardiology and Cardiovascular Interventions’ I am sincerely grateful to the coordinated work of the journal team for the no problem with the submission of my manuscript: “Cardiometabolic Disorders in A Pregnant Woman with Severe Preeclampsia on the Background of Morbid Obesity (Case Report).” The review process by 5 experts was fast, and the comments were professional, which made it more specific and academic, and the process of publication and presentation of the article was excellent. I recommend that my colleagues publish articles in this journal, and I am interested in further scientific cooperation. Sincerely and best wishes, Dr. Oleg Golyanovskiy.
