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Research Article | DOI: https://doi.org/10.31579/2690-1919/570
Assistant Professor, Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India.
*Corresponding Author: Alagbe John Olujimi, Assistant Professor, Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India.
Citation: Alagbe J. Olujimi, (2025), Effect of Dietary Supplementation of Asparagus racemosus Essential oil on Some Haemato-Biochemical Constituents and Oxidative Stress Indices of Gaddi Rams, J Clinical Research and Reports, 21(2); DOI:10.31579/2690-1919/570
Copyright: © 2025, Alagbe John Olujimi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 16 August 2025 | Accepted: 11 September 2025 | Published: 22 September 2025
Keywords: Asparagus racemosus; essential oil; haematology; serum; oxidative stress
This experiment was designed to determine the effect of dietary supplementation of Asparagus racemosus essential oil on some haemato-biochemical constituents and oxidative stress indices of Gaddi rams. Twenty-four male Gaddi rams with a mean body weight of 32.00 ± 1.8 kg and an average age of 7 months were used in a completely randomized design for a 60-day feeding period preceded by a 14-day quarantine period. Experimental treatments included: treatment 1 (control) – basal diet without essential oil; treatment 2 and treatment 3 – basal diet supplemented with 200 mg and 400 mg Asparagus racemosus essential oil per kg DM feed daily. Fresh water was supplied ad libitum and all other management practices were strictly observed. Results obtained revealed that Asparagus racemosus essential oil contained several bioactive compounds with therapeutic properties. Asparagus racemosus essential oil supplementation enhanced the level of pack cell volume, haemoglobin, red blood cell, lymphocytes, eosinophils and monocytes (p˂0.05). The serum levels of total protein, albumin, globulin, glucose, calcium, phosphorus, potassium, sodium, chloride and bicarbonate concentrations (p˂0.05). Conversely, concentrations of creatinine, urea, alanine amino transferase and aspartate transaminase were not affected (p˃0.05). Asparagus racemosus essential oil supplementation decreased malondialdehyde concentrations in the blood and increased the levels (P < 0.05) of catalase, superoxide dismutase and glutathione peroxidase. It was concluded that Asparagus racemosus essential oil dietary supplementation up to 400 mg per kg DM feed did not pose any negative effect on the health status of animals.
Medicinal plants have the capability to synthesize secondary metabolites or phytochemicals which confers them the ability to perform several pharmacological activities [1]. These compounds have been generally regarded as safe, efficient, capable of reducing environmental pollution and have no withdrawal period [2,3]. Essential oils are plant extracts obtained through extraction with organic solvents and subsequent distillation. These extracts are a combination of different molecules because the way they are extracted will carry a general group of chemicals with specific characteristics. Essential oils have also been considered as safe alternative to antibiotics because they do not cause antimicrobial resistance nor do they create toxic residues in animal products [4]. Among the potential essential oils with therapeutic relevance are those from Asparagus racemosus.
Asparagus racemosus is a shrub with a tuberous root belonging to the family Asparagaceae. The genus Asparagus includes about 300 species widely distributed globally. The plant is found at low altitudes in shade and in tropical climates including Africa, Europe, Asia including India [5,6]. Results from previous investigation into the chemical composition of Asparagus racemosus leaf, stem and root showed that the investigated samples contain phytochemicals such as polyphenols, saponins, tannins, steroids, alkaloids and flavonoids which have antiplatelet, cytotoxicity, angiogenic, antitumor, antineoplasic, antiviral, sedative, muscle relaxant, cytotoxic, antiviral, insecticidal, cardiotonic, analgesic, inhibition of lipid peroxidation, anti-inflammatory, fungicidal, antiprotozoal, antimalarial, and antirheumatic, Antifertility, antinociceptive, antidiarrhoea, antifungal, immuno-stimulatory, anti-tumor, hepato-protective, amongst others [7]. Chemical analysis of Asparagus racemosus revealed that the leaf has several nutritional benefits and it is loaded with vitamins such as vitamin A, B1, B2, C, E, Mg, P, Ca, Fe, and folic acid [8]. It's essential oil also contains important amino acid, asparagine, arginine, tyrosine and valine [9]. Asparagus racemosus leaf decoction is used to treat diarrhea, hypertension, gastro intestinal problems, malaria, skin infection, asthmas, cough, bronchitis, and expectorant [10]. Essential oil from the leaves have also been confirmed to inhibit the activities of some pathogenic organisms [11].
It has been well-documented that dietary supplementation of essential oils could improve animal performance by increasing digestive enzyme secretion, lowering the number of harmful bacteria in the digestive tract, modulating intestinal morphology functions, and positively affecting productivity, blood metabolites, and immunological and antioxidant status of ruminants. [12,13]. Supplementation of thyme oil in the diet of sheep has been reported to confer positive immune effects such as increase in lymphocyte proliferation rate, phagocytic rate as well as increase in immunoglobulins in the blood of goats [14].
This research is timely because the demand for natural antioxidants in animal feed is increasing due to their health benefits against oxidative stress and several diseases. A timely evaluation of the bioactive compounds in Asparagus racemosus will further give insight their therapeutic properties and also use it to address the high rate of antimicrobial resistance and promote food safety.
Study Area
The study was carried out at the Sheep and Goat section, Ganhi College of Agriculture located at Rajasthan, India. All animals used in the experiment were cared for according to applicable recommendations of Indian Society of Animal Production. All laboratory analysis was carried out at Sumitra Research Institute, Gujarat India using standard procedures according to Association of Analytical Chemist.
Animals, experimental design, and sampling
Twenty-four male Gaddi rams with a mean body weight of 32.00 ± 1.8 kg and an average age of 7 months were used in a completely randomized design for a 60-day feeding period preceded by a 14-day quarantine period. During the adaptation period, goats were vaccinated against Peste Des Petits and dewormed using Wormzap Albendazole®. Animals were weighted at the end of the quarantine period using digital sensitive scale and stratified based on their weights into three dietary groups with 8 replicates each. Goats were housed in individual pens measuring (2m2/goat) equipped with feeders and drinkers. Experimental treatments included: treatment 1 (control) – basal diet without essential oil; treatment 2 and treatment 3 – basal diet supplemented with 200 mg and 400 mg Asparagus racemosus essential oil per kg DM feed daily. The basal diet was formulated to meet the standard nutrient requirements of goats according to Nutritional Research Council recommendations [15]. Before the morning feeding, 200 g total diet DM was mixed with Asparagus racemosus essential oil in each treatment to ensure the consumption of their full dose. Animals were fed thrice daily between at 07:00, 12:00 and 16:00 h and had unrestricted access to clean water. Cleaning of the pens and washing of drinkers was done daily. During the experimental period, goats were weighed individually weekly. The amount of both offered and refusal feed were recorded every day to determine their feed intake. Composition of Asparagus racemosus essential oil and experimental diet is presented in Table 1 and 2 respectively.
Asparagus racemosus oil extraction and Gas chromatography-mass spectrometry analysis
Fresh leaves of Asparagus racemosus were sourced from the premises of Gandhi College of Agriculture, Rajasthan in India. The collected leaves were taken to the Taxonomy Department, Sumitra Research Institute, Rajasthan for identification before it was assigned an authentication number – SRI-00T/2024. The leaves were rinsed in a plastic of water to remove dirt’s, spread on a plastic sieve for 20 minutes to allow water drain and air dried for eight days. Dried samples were later pulverized into powder using a multi-purpose electric blender before extraction. Extraction of oil was done using steam distillation technique with Clevenger apparatus were recently published by [16]. The oil obtained was stored in the refrigerator at a temperature of 4 °C prior to laboratory analysis.
Analysis of Asparagus racemosus oil was performed on an Agilent Model 7890A Gas Chromatography interfaced to an Agilent 7000 GC/MS Triple Quad. The equipment carrier gas was helium which was maintained at a pressure, temperature and average velocity of 1.500 psi, 300 °C and 43.11 cm/sec respectively to maintain accuracy according to the manufacturers recommendation in the GC. The MS was operated at an ionization voltage of 70eV with ion source temperature of 230 °C, quadrupole temperature of 150 °C. Components identification The components of the essential oil were identified on the basis of their retention indices. Identification confirmation of reference compounds from the Library of National Institute of Standard and Technology database.
Collection and analysis of blood constituents
On the last day of the experiment, 10 mL of blood was collected from the jugular vein of four animals per treatment into two bottles (5 mL each). Samples for haematology were collected into bottles with anticoagulant while those for serum were put into plain bottles. Collected samples were placed in an ice pack before transporting them to the laboratory. Heamatological parameters were analyzed using Mission® HA-360 3 – automatic haemo-analyzer (Netherlands) which uses cyanide free reagent. White blood cell count is determined through impedence method, red blood cell (sheath flow impedence method), haemoglobin count (colorimetric method) and the machine is adjusted to a temperature of 15 – 30 ◦C and humidity of 50 – 80 % for proper precision once the samples were arranged in the collection chamber.
Serum parameters were analyzed using SKLA-HB1-POC auto-chemistry analyzer (Taiwan). Kit is adjusted to a sample volume of 200 µL, temperature / humidity of 10 to 32 ◦C/ 50 to 90 % to ensure accuracy in the results.
Determination of oxidative stress indices
Malondialdehyde, superoxide dismutase, catalase and glutathione peroxidase were analyzed using the samples for haematological studies. Fully automatic chemiluminscence immuno-analyzer (YSTE-CF10, Netherlands) adjusted to a reaction temperature of 37◦C, relative humidity (30 to 65 %) and a measuring time of up to 20 minutes for 12 simultaneous samples.
All data collected were subjected to a one-way analysis of variance (ANOVA). Means showing significant differences were separated using Duncan’s Multiple Range Test. The SPSS (version 25) statistical package was used for all statistical analysis.
| S/N | Compounds | % Concentration | R.T (min) |
| 1 | Octadecanoic acid | 0.10 | 6.00 |
| 2 | 2-methyloctacosane | 0.16 | 6.24 |
| 3 | Ethyl ricinoleate | 0.08 | 7.08 |
| 4 | Hexahydrofarnesyl acetone | 0.11 | 7.37 |
| 5 | Hexadecanoic acid | 0.17 | 7.55 |
| 6 | Tritetracontane | 0.02 | 8.01 |
| 7 | 1-Butene,4-iodo | 0.04 | 8.85 |
| 8 | 1,2,3-Propanetriol,1- acetate | 0.19 | 8.97 |
| 9 | Phytol | 28.40 | 9.11 |
| 10 | Squalene | 2.19 | 10.10 |
| 11 | 1-Octanol, 2-butyl | 0.01 | 10.37 |
| 12 | Oleic acid | 0.08 | 10.89 |
| 13 | Trans-9-Octadecenoic acid, pentyl ester | 0.13 | 11.51 |
| 14 | 9-Tetradecenal | 0.02 | 11.90 |
| 15 | Octadecanoic acid | 0.18 | 12.33 |
| 16 | Chloromethyl 7-chlorododecanoate | 0.02 | 12.91 |
| 17 | Stigmasterol | 0.09 | 13.00 |
| 18 | Ethyl ricinoleate | 0.25 | 13.26 |
| 19 | Linoleic acid | 0.16 | 13.79 |
| 20 | Butyl tetratriacontyl ether | 0.02 | 14.02 |
| 21 | Ethyl ricinoleate | 0.06 | 15.02 |
| 22 | 2-Dimethylsilyloxytetradecane | 8.71 | 15.28 |
| 23 | 2-Furanone,3,4- dihydroxytetrahydro | 0.04 | 15.81 |
| 24 | Cyclopentanedione | 10.41 | 16.09 |
| 25 | IsosorbideDinitrate | 9.96 | 17.11 |
| 26 | Ethyliso-allocholate | 0.15 | 18.05 |
| 27 | 4H-Pyran-4-one,2,3- dihydro-3,5-dihydroxy-6-methyl 2(3H)- | 10.90 | 18.39 |
| 28 | 9-Eicosyne | 8.21 | 18.55 |
| 29 | Phenol,4,4'-(3- ethenyl-1-propene-1,3-diyl)bis-,(E) | 12.04 | 18.92 |
| 30 | 7- Dehydrodiosgenin3- acetate | 0.05 | 19.07 |
| 31 | 3-Deoxy-d-mannoiclactone | 0.17 | 19.55 |
| 32 | Quinoline,8- hydrazine | 0.10 | 20.40 |
| Total | 93.22 |
Table 1: Chemical composition of Asparagus racemosus oil
| Components | Quantity (g/kg DM) |
| Maize (890 g/kg CP) | 300.0 |
| Corn barn | 260.0 |
| Cowpea husk | 200.0 |
| Groundnut cake (400 g/kg CP) | 135.0 |
| Soya bean meal (480 g/kg CP) | 100.0 |
| Growers Mineral/Vitamin Premix | 2.00 |
| Limestone | 2.00 |
| Salt | 1.00 |
| Total | 1000.0 |
| Chemical composition (g/kg dry matter - DM) | |
| Dry matter | 940.0 |
| Organic matter | 930.0 |
| Crude protein | 157.0 |
| Ether extract | 30.00 |
| Ash | 70.00 |
| Non-structural carbohydrate | 350.00 |
| Acid detergent fibre | 220.00 |
| Acid detergent lignin | 300.00 |
| Neutral detergent fibre | 371.00 |
| Cellulose | 190.7 |
| Hemicellulose | 15.00 |
| Energy (Kcal/kg) | 2600.8s |
Table 2: Ingredient and chemical composition of basal diet (g/kg of DM)
2.5 kg Growers premix contained: 15,000 I.U. vitamin A; 8000 mg vitamin B1; 3000 I.U. vitamin D3; 60.0 mg vitamin E; 15 mg Choline; 0.96 mg Cobalt; 2.00 mg I; 50 manganese Mn; 0.50 mg Selenium; 250 mg.
| Constituents | T1 | T2 | T3 | SEM |
| Pack cell volume (%) | 29.74b | 32.08a | 33.12a | 2.69 |
| Red blood cell (×106/L) | 6.96b | 9.03a | 9.11a | 0.18 |
| Haemoglobin (g/dL) | 8.92b | 10.40a | 10.95a | 0.25 |
| White blood cell (×103/L) | 5.73b | 7.19a | 7.63a | 0.16 |
| Lymphocytes (%) | 50.09b | 67.99a | 68.05a | 4.08 |
| Monocytes (%) | 1.25b | 2.10a | 2.18a | 0.01 |
| Eosinophils (%) | 3.91b | 4.03a | 4.15a | 0.02 |
a,b: Means within a row with different superscripts are significantly different (p˂0.05); T1: basal diet without oil; T2 and T3: basal diet with supplemented with 200 mg and 400 mg Asparagus racemosus essential oil per kg DM feed daily
Table 3: Haematological parameters of Gaddi rams fed diet supplemented with Asparagus racemosus oil
| Constituents | T1 | T2 | T3 | SEM |
| Total protein (g/dL) | 4.01b | 5.18a | 5.36a | 0.02 |
| Albumin (g/dL) | 2.06b | 2.98a | 3.20a | 0.01 |
| Globulin (g/dL) | 1.95b | 2.20a | 2.16a | 0.01 |
| Urea (mg/dL) | 6.62 | 6.51 | 6.48 | 0.15 |
| Creatinine (mg/dL) | 4.02 | 4.00 | 3.98 | 0.10 |
| Glucose (mg/dL) | 50.90b | 65.17a | 66.02a | 3.07 |
| Cholesterol (mg/dL) | 55.02a | 47.62b | 46.61b | 2.05 |
a,b: Means within a row with different superscripts are significantly different (p˂0.05); T1: basal diet without oil; T2 and T3: basal diet with supplemented with 200 mg and 400 mg Asparagus racemosus essential oil per kg DM feed daily
Table 4: Serum biochemical indices of Gaddi rams fed diet supplemented with Asparagus racemosus oil
| Constituents | T1 | T2 | T3 | SEM |
| ALT (U/L) | 62.19 | 65.80 | 64.19 | 2.63 |
| AST (U/L) | 30.67 | 31.22 | 31.83 | 1.85 |
| Calcium (mmol/L) | 1.82b | 2.96a | 2.98a | 0.02 |
| Phosphorus (mmol/L) | 1.00b | 1.68a | 1.70a | 0.10 |
| Potassium (mmol/L) | 2.40b | 3.27a | 3.30a | 0.11 |
| Sodium (mmol/L) | 118.5b | 131.9a | 135.6a | 8.94 |
| Chloride (mmol/L) | 100.6b | 118.7a | 121.1a | 7.06 |
| Bicarbonate (mmol/L) | 61.55b | 90.31a | 90.88a | 3.77 |
a,b: Means within a row with different superscripts are significantly different (p˂0.05) ; ALT: alanine amino transferase; AST: aspartate transaminase; T1: basal diet without oil; T2 and T3: basal diet with supplemented with 200 mg and 400 mg Asparagus racemosus essential oil per kg DM feed daily
Table 5: Serum enzymes and ions of Gaddi rams fed diet supplemented with Asparagus racemosus oil
| Parameters | T1 | T2 | T3 | SEM |
| Superoxide dismutase (ng/mL) | 20.85b | 33.86a | 34.07a | 2.30 |
| Glutathione peroxidase (ng/mL) | 19.05b | 25.06a | 25.14a | 1.69 |
| Malondialdehyde (ng/mL) | 106.5a | 98.05b | 97.12b | 6.32 |
| Catalase (µmol/L) | 40.08b | 54.18a | 55.02a | 4.11 |
a,b: Means within a row with different superscripts are significantly different (p˂0.05); T1: basal diet without oil; T2 and T3: basal diet with supplemented with 200 mg and 400 mg Asparagus racemosus essential oil per kg DM feed daily
Table 6: Oxidative stress parameters of Gaddi rams fed diet supplemented with Asparagus racemosus oil
Asparagus racemosus oil GC-MS analysis (Table 2) showed the total of 32 bioactive compounds representing 93.22 %. The major constituents identified were; Phytol (28.40 %), Phenol,4,4'-(3- ethenyl-1-propene-1,3-diyl) bis-, (E) (12.04 %), 4H-Pyran-4-one,2,3- dihydro-3,5-dihydroxy-6-methyl 2(3H)- (10.90 %), Cyclopentanedione (10.41 %), IsosorbideDinitrate (9.96 %), 2-Dimethylsilyloxytetradecane (8.71 %) and 9-Eicosyne (8.21 %) while Chloromethyl 7-chlorododecanoate (0.02 %), Butyl tetratriacontyl ether (0.02 %), Tritetracontane (0.02 %) and 1-Octanol, 2-butyl (0.01 %) were amongst the minor compounds. The result obtained in this study is in agreement with earlier reports on the root extract of Asparagus racemosus [18, 19] with respect to the presence of Phytol, 2-Furanone,3,4- dihydroxytetrahydro and 2-Dimethylsilyloxytetradecane. However, all compounds identified in the sample has several pharmacological activities including, antimicrobial [20], anti-inflammatory, hypercholesterolemic [21], nematicide, anti-cancer, anti-tumor [22], hepato-protective [23], Insectifuge, anti-staminic, analgesics [24], 5 alpha reductase inhibitor, anti-eczemic, anti-androgenic, anti-coronary [25], anti-arthritis, antioxidant, anti-androgenic, hemolytic, dermatitigenic, anti-helminthic [26,27], immuno-modulatory [27], antidiarrheal, antifungal, antiviral amongst others.
Haematological parameters of Gaddi rams fed diet supplemented with Asparagus racemosus oil (Table 3). Pack cell volume, haemoglobin and red blood cell values were lower in treatment 1 {(29.74 %, 8.92 g/dL and 6.96 (×106/L)} than treatment 2 {(32.08 %, 10.40 g/dL, 9.03 (×106/L)} and treatment 3 {(33.12 %, 10.95 g/dL, 9.11 (×106/L)} (p˂0.05). Pack cell volume recorded in this study was within (29.00 – 36.00 %) cited by [28]. Haemoglobin and red blood cell values were within 6.90 – 16.00 g/dL and 5.80 – 15.00 (×106/L) reported by [29]. This outcome suggests that Asparagus racemosus oil dietary supplementation could enhance the production of iron to make haemoglobin which allows the efficient transportation of oxygen to the lungs and other parts of the body [30]. White blood cell {(5.73 – 7.63 (×103/L)}, lymphocytes {(50.09 – 68.05 %)}, monocytes {(1.25 – 2.18 %)} and eosinophils {(3.91 – 4.15 %)} in treatment 2 and 3 were similar (p˃0.05) but significantly higher than those in treatment 1 (p˂0.05). This result suggests that Asparagus racemosus oil has antioxidant capacity thus increasing the phagocytic rate as well as the proliferation of lymphocytes and immunoglobulin to prevent infection in the body of animals [28]. White blood cell, lymphocytes, monocytes and eosinophil counts were within the reference interval cited by [29]. [30] recorded a lower white blood cell count of {(4.91 – 6.12 (×103/L)}in calves fed different levels of thyme and cinnamon essential oil. This variation could be as a result of influence of bioactive compounds, extraction method, differences in dosage used as well as duration of experiment [31].
Table 4 reveals the serum biochemical indices of Gaddi rams fed diet supplemented with Asparagus racemosus oil. Total protein (4.01 – 5.36 g/dL), albumin (2.06 – 3.20 g/dL) and globulin values (1.95 – 2.16 g/dL) were influenced by the treatment (p˂0.05). Total protein value obtained in this study is in agreement with the normal range (4.00 – 8.25 g/dL) reported by [32]. This result suggests that dietary protein in the experimental diet was sufficient for growth and development of animals [25]. Urea and creatinine values which ranged from 6.62 to 6.48 mg/dL and 3.98 to 4.02 mg/dL were not affected (p˃0.05) by the treatment. However, values recorded in this experiment were within the normal level [(urea: 3.00 – 8.00 mg/dL)] and [(creatinine: 2.30 – 5.06 mg/dL) reported by [33]. This result is a clear sign that the kidneys of the animals were in good state indicating that supplementing Asparagus racemosus oil at 400 mg/kg in the diet of sheep was not detrimental to their body. Glucose and cholesterol values were consistent with the findings of [27] who recorded a normal range of 43.19 – 78.00 mg/dL and 50.60 – 110.0 mg/dL respectively. Cholesterol level declines as the level of Asparagus racemosus oil increases across the treatment indicating that it possesses hypo-lipidemic properties [26].
Serum enzymes and ions of Gaddi rams fed diet supplemented with Asparagus racemosus oil (Table 5). Calcium (1.82 – 2.92 mmol/L), phosphorus (1.00 – 1.70 mmol/L), potassium (2.40 – 3.30 mmol/L), sodium (118.5 – 135.6 mmol/L), chloride (100.6 -121.1 mmol/L) and bicarbonate (61.55 – 90.88 mmol/L) follow similar pattern and were higher in treatment 3 and 2 than in treatment 1 (p˂0.05). Values obtained in this study were consistent with the findings of [34]. This outcome suggests sufficient concentrations of serum minerals needed to maintain fluid and pH balance to ensure proper functioning of the cells and nerves of animals [24]. Alanine amino transferase and aspartate transaminase values [(62.19 – 64.19 U/L; 30.67 – 31.83 U/L)] were not significantly affected (p˃0.05) by the treatment. However, values obtained were in agreement with the normal range reported by [33, 37]. This result indicates the absence of hepatic disorder in the body of animals [25, 36].
Oxidative stress parameters of Gaddi rams fed diet supplemented with Asparagus racemosus oil (Table 6). Superoxide dismutase, glutathione peroxidase and catalase values were lower in treatment 1 [(20.85 ng/mL; 19.05 ng/mL and 40.08 (µmol/L)] than in treatment 2 [(33.86 ng/mL; 25.06 ng/mL and 54.18 (µmol/L)] and [(34.07 ng/mL; 25.14 ng/mL and 55.02 (µmol/L)] (p˂0.05). Conversely, malondialdehyde values were higher in treatment 1 (106.5 ng/mL) than in treatment 2 (98.05 ng/mL) and 3 (97.12 ng/mL) (p˂0.05). This result suggests that supplementing Asparagus racemosus oil in the diet of animals is capable of neutralizing malondialdehyde by scavenging free radicals capable of causing disease and increased production of superoxide dismutase and glutathione peroxidase due to the presence of bioactive compounds (Table 1). The result obtained is in agreement with the report of [38] when rosemary oil was supplemented in the diet of fattening lambs.
In conclusion, Asparagus racemosus oil contains several bioactive compounds with therapeutic properties. Supplementing Asparagus racemosus oil up to 400 mg per kg DM feed daily improved phygocytic rate, blood constituents and scavenge the activities of free radicals thus helping animal to maintain a stable health status and resistance against disease.
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Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.
Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.
I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!
"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".
I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.
We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.
I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.
I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.
I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.
Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.
“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.
Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.
My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.
My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.
I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.
Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."
I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.
To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.
"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".
I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.
Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.
We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.
My article, titled 'No Way Out of the Smartphone Epidemic Without Considering the Insights of Brain Research,' has been republished in the International Journal of Clinical Case Reports and Reviews. The review process was seamless and professional, with the editors being both friendly and supportive. I am deeply grateful for their efforts.
To Dear Erin Aust – Editorial Coordinator of Journal of General Medicine and Clinical Practice! I declare that I am absolutely satisfied with your work carried out with great competence in following the manuscript during the various stages from its receipt, during the revision process to the final acceptance for publication. Thank Prof. Elvira Farina
Dear Jessica, and the super professional team of the ‘Clinical Cardiology and Cardiovascular Interventions’ I am sincerely grateful to the coordinated work of the journal team for the no problem with the submission of my manuscript: “Cardiometabolic Disorders in A Pregnant Woman with Severe Preeclampsia on the Background of Morbid Obesity (Case Report).” The review process by 5 experts was fast, and the comments were professional, which made it more specific and academic, and the process of publication and presentation of the article was excellent. I recommend that my colleagues publish articles in this journal, and I am interested in further scientific cooperation. Sincerely and best wishes, Dr. Oleg Golyanovskiy.
Dear Ashley Rosa, Editorial Coordinator of the journal - Psychology and Mental Health Care. " The process of obtaining publication of my article in the Psychology and Mental Health Journal was positive in all areas. The peer review process resulted in a number of valuable comments, the editorial process was collaborative and timely, and the quality of this journal has been quickly noticed, resulting in alternative journals contacting me to publish with them." Warm regards, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. I appreciate the journal (JCCI) editorial office support, the entire team leads were always ready to help, not only on technical front but also on thorough process. Also, I should thank dear reviewers’ attention to detail and creative approach to teach me and bring new insights by their comments. Surely, more discussions and introduction of other hemodynamic devices would provide better prevention and management of shock states. Your efforts and dedication in presenting educational materials in this journal are commendable. Best wishes from, Farahnaz Fallahian.
Dear Maria Emerson, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. I am delighted to have published our manuscript, "Acute Colonic Pseudo-Obstruction (ACPO): A rare but serious complication following caesarean section." I want to thank the editorial team, especially Maria Emerson, for their prompt review of the manuscript, quick responses to queries, and overall support. Yours sincerely Dr. Victor Olagundoye.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. Many thanks for publishing this manuscript after I lost confidence the editors were most helpful, more than other journals Best wishes from, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Agrippa Hilda, Editorial Coordinator, Journal of Neuroscience and Neurological Surgery. The entire process including article submission, review, revision, and publication was extremely easy. The journal editor was prompt and helpful, and the reviewers contributed to the quality of the paper. Thank you so much! Eric Nussbaum, MD
Dr Hala Al Shaikh This is to acknowledge that the peer review process for the article ’ A Novel Gnrh1 Gene Mutation in Four Omani Male Siblings, Presentation and Management ’ sent to the International Journal of Clinical Case Reports and Reviews was quick and smooth. The editorial office was prompt with easy communication.
Dear Erin Aust, Editorial Coordinator, Journal of General Medicine and Clinical Practice. We are pleased to share our experience with the “Journal of General Medicine and Clinical Practice”, following the successful publication of our article. The peer review process was thorough and constructive, helping to improve the clarity and quality of the manuscript. We are especially thankful to Ms. Erin Aust, the Editorial Coordinator, for her prompt communication and continuous support throughout the process. Her professionalism ensured a smooth and efficient publication experience. The journal upholds high editorial standards, and we highly recommend it to fellow researchers seeking a credible platform for their work. Best wishes By, Dr. Rakhi Mishra.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. The peer review process of the journal of Clinical Cardiology and Cardiovascular Interventions was excellent and fast, as was the support of the editorial office and the quality of the journal. Kind regards Walter F. Riesen Prof. Dr. Dr. h.c. Walter F. Riesen.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. Thank you for publishing our article, Exploring Clozapine's Efficacy in Managing Aggression: A Multiple Single-Case Study in Forensic Psychiatry in the international journal of clinical case reports and reviews. We found the peer review process very professional and efficient. The comments were constructive, and the whole process was efficient. On behalf of the co-authors, I would like to thank you for publishing this article. With regards, Dr. Jelle R. Lettinga.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, I would like to express my deep admiration for the exceptional professionalism demonstrated by your journal. I am thoroughly impressed by the speed of the editorial process, the substantive and insightful reviews, and the meticulous preparation of the manuscript for publication. Additionally, I greatly appreciate the courteous and immediate responses from your editorial office to all my inquiries. Best Regards, Dariusz Ziora
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation, Auctores Publishing LLC, We would like to thank the editorial team for the smooth and high-quality communication leading up to the publication of our article in the Journal of Neurodegeneration and Neurorehabilitation. The reviewers have extensive knowledge in the field, and their relevant questions helped to add value to our publication. Kind regards, Dr. Ravi Shrivastava.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, Auctores Publishing LLC, USA Office: +1-(302)-520-2644. I would like to express my sincere appreciation for the efficient and professional handling of my case report by the ‘Journal of Clinical Case Reports and Studies’. The peer review process was not only fast but also highly constructive—the reviewers’ comments were clear, relevant, and greatly helped me improve the quality and clarity of my manuscript. I also received excellent support from the editorial office throughout the process. Communication was smooth and timely, and I felt well guided at every stage, from submission to publication. The overall quality and rigor of the journal are truly commendable. I am pleased to have published my work with Journal of Clinical Case Reports and Studies, and I look forward to future opportunities for collaboration. Sincerely, Aline Tollet, UCLouvain.
Dear Ms. Mayra Duenas, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. “The International Journal of Clinical Case Reports and Reviews represented the “ideal house” to share with the research community a first experience with the use of the Simeox device for speech rehabilitation. High scientific reputation and attractive website communication were first determinants for the selection of this Journal, and the following submission process exceeded expectations: fast but highly professional peer review, great support by the editorial office, elegant graphic layout. Exactly what a dynamic research team - also composed by allied professionals - needs!" From, Chiara Beccaluva, PT - Italy.
Dear Maria Emerson, Editorial Coordinator, we have deeply appreciated the professionalism demonstrated by the International Journal of Clinical Case Reports and Reviews. The reviewers have extensive knowledge of our field and have been very efficient and fast in supporting the process. I am really looking forward to further collaboration. Thanks. Best regards, Dr. Claudio Ligresti
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation. “The peer review process was efficient and constructive, and the editorial office provided excellent communication and support throughout. The journal ensures scientific rigor and high editorial standards, while also offering a smooth and timely publication process. We sincerely appreciate the work of the editorial team in facilitating the dissemination of innovative approaches such as the Bonori Method.” Best regards, Dr. Matteo Bonori.
I recommend without hesitation submitting relevant papers on medical decision making to the International Journal of Clinical Case Reports and Reviews. I am very grateful to the editorial staff. Maria Emerson was a pleasure to communicate with. The time from submission to publication was an extremely short 3 weeks. The editorial staff submitted the paper to three reviewers. Two of the reviewers commented positively on the value of publishing the paper. The editorial staff quickly recognized the third reviewer’s comments as an unjust attempt to reject the paper. I revised the paper as recommended by the first two reviewers.
Dear Maria Emerson, Editorial Coordinator, Journal of Clinical Research and Reports. Thank you for publishing our case report: "Clinical Case of Effective Fetal Stem Cells Treatment in a Patient with Autism Spectrum Disorder" within the "Journal of Clinical Research and Reports" being submitted by the team of EmCell doctors from Kyiv, Ukraine. We much appreciate a professional and transparent peer-review process from Auctores. All research Doctors are so grateful to your Editorial Office and Auctores Publishing support! I amiably wish our article publication maintained a top quality of your International Scientific Journal. My best wishes for a prosperity of the Journal of Clinical Research and Reports. Hope our scientific relationship and cooperation will remain long lasting. Thank you very much indeed. Kind regards, Dr. Andriy Sinelnyk Cell Therapy Center EmCell
Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. It was truly a rewarding experience to work with the journal “Clinical Cardiology and Cardiovascular Interventions”. The peer review process was insightful and encouraging, helping us refine our work to a higher standard. The editorial office offered exceptional support with prompt and thoughtful communication. I highly value the journal’s role in promoting scientific advancement and am honored to be part of it. Best regards, Meng-Jou Lee, MD, Department of Anesthesiology, National Taiwan University Hospital.
