Research Article | DOI: https://doi.org/10.31579/2637-8892/006
*Corresponding Author: Garrepalli samatha, Department of Pharmaceutics, Bomma college pharmacy, India.
Citation: Garrepalli samatha and Uma Rani. G, Anti-Inflammatory Activity: Assessment of Benzo [6, 7] Cyclohepta [1, 2-b] Pyridine Derivatives. J Psychology and Mental Health Care. 1(2); DOI: 10.31579/2637-8892/006
Copyright: © 2017 Garrepalli samatha. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Received: 01 June 2017 | Accepted: 09 June 2017 | Published: 21 June 2017
Keywords: benzosuberone; benzo [6,7] cyclohepta [1,2-b] pyridines; anti-proliferative; cell lines; doxorubicin; paclitaxel
A variety of benzo [6,7] cyclohepta [1,2-b] pyridine derivatives have been evaluated for their in vitro anti-proliferative activity against four cancer cell lines such as HeLa (cervical), MIAPACA (pancreatic), MDA-MB-231 (breast) and IMR32 (neuroblastoma). Among tested compounds, a exhibited potent antiproliferative activity against IMR32 with GI50 value less than 0.01 μM and compounds d, l and n exhibited promising anti-proliferative activity against IMR32 with GI50 values 0.1, 0.21 and 0.21 μM, respectively. This is the first report on in vitro anti-proliferative evaluation of benzo [6,7] cyclohepta [1,2-b] pyridine derivatives in addition to anti-inflammatory activity.
Benzosuberone motif has remarkable biological activities such as anti-inflammatory, anti-pyretic, anti-ulcer, CNS-depressant, CNS stimulant and anticonvulsant activities. And some of the derivativesare also known for anti-tumor activity in murine p338 cell line tests [1]. In addition these derivatives widely used in pharmaceutical applications, such as tricyclic antidepressants containingdibenzosuberone moieties mostly effect the autonomic and central nervous systems, and traditional anti-depressants, like amitriptyline [2], imipramine [3] and noxiptiline [4] which continue to be used as first-line drugs in treating depressive disorders.
Pyridyl compounds are of interest to organic chemists in recent years owing to their wide spectrum of physiological activity [5-10]. The condensed derivatives of pyridines play significant role in bioactive molecules, especially in the form of benzo [5,6] cyclohepta [1,2-b] pyridines which are structural analogues to benzosuberone. The benzo [5,6] cyclohepta [1,2-b] pyridine is an important core biologically active compound with diverse biological activities, such as antihistamine as well as antitumor and antiinflammatory activities [11-17]. It is a highly potent pharmacophore and widely used in drug molecular design. Because of the important aforementioned properties of benzocyclohepta [1,2-b] pyridines derivatives, preparation of this heterocyclic nucleus has gained great importance in organic synthesis.
Recently, we have published [18] synthesis and anti-inflammatory activity of benzo [6,7] cyclohepta [1,2-b] pyridines, in the present manuscript, presenting in vitro anti-proliferative activity of benzo [6,7] cyclohepta [1,2-b] pyridines against four cancer cell lines such as HeLa (cervical), MIAPACA (pancreatic), MDA-MB-231 (breast) and IMR32 (neuroblastoma) and a SRB cell proliferation assay to estimate viability or growth in addition to anti-inflammatory activity in continuation to our ongoing research activities [9-27], to discover and develop tumor growth inhibitors and apoptotic inducers as potential new anti-cancer agents.
Effects of the Compounds on the Viability of Human Cancer Cells
All the synthesized compounds of benzo [6,7] cyclohepta [1,2-b] pyridine derivatives (a-n) were screened for their in vitro antiproliferative activity against four cancer cell lines such as HeLa(cervical), MIAPACA (pancreatic), MDA-MB-231 (breast) and IMR32 (neuroblastoma) .
The compounds were picked for an advanced assay against these four human cancer cell lines at five concentrations (0.01, 0.1, 1, 10, 100 μM). GI50 (growth inhibitory activity) was calculated and these values corresponded to the concentration of the compound causing 50
Taken together, we have synthesized a series of benzo [6,7]cyclohepta [1,2-b] pyridine derivatives (a-n) in good yields and performed there in vitro anti-proliferative activity against four different human cancer cell lines, HeLa, MIAPACA, MDA-MB-231 and IMR32. Compound a exhibited potent anti-proliferative activity against IMR32 with GI50 value less than 0.01 μM, which was more active than standards Doxorubicin and Paclitaxel in addition to anti-inflammatory activity.
Dear Grace Pierce, Editorial Coordinator of Journal of Clinical Research and Reports, Thank you for the speedy and efficient peer review process. I appreciate the fact that your peer reviewers do not take months to respond like with some other journals. I would also like to thank the editorial office for responding quickly to my questions. It is an excellent journal. I plan to submit more manuscripts in the future. Best wishes from, Robert W. McGee
Dear Grace Pierce, Editorial Coordinator of Journal of Clinical Research and Reports, Working with you and your team on our recent publication in JCRR has been a truly wonderful and enjoyable experience. The responses were prompt, and the reviewers were patient, constructive, and highly professional. One reviewer in particular gave me the feeling that a professor was carefully reading and commenting on my coursework, which was deeply touching. The entire process was straightforward and hassle‑free, with no tedious online forms to complete. I highly recommend this journal. Best wishes from, DR Aibing Rao, Head of R&D
I Appreciate the Opportunity to Share my Experience with the Journal of Clinical Research and Reports. The peer review process was timely and constructive, and the feedback provided helped improve the quality of our manuscript. The editorial office was professional, responsive, and supportive throughout the process, ensuring smooth communication and efficient handling of the submission. Overall, it was a positive experience collaborating with your team.
Dear Mercy Grace, Editorial Coordinator of Obstetrics Gynecology and Reproductive Sciences, We would like to express our gratitude for your help at all stages of publishing and editing the article. The editors of the magazine answer all the necessary questions and help at every stage. We will definitely continue to cooperate and publish other works in the Obstetrics Gynecology and Reproductive Sciences! Best wishes from, Alla Konstantinovna Politova,
Dear Maria Emerson, Editorial Coordinator of International Journal of Clinical Case Reports and Reviews, What distinguishes International Journal of Clinical Case Report and Review is not only the scientific rigor of its publications, but the intellectual climate in which research is evaluated. The submission process is refreshingly free of unnecessary formal barriers and bureaucratic rituals that often complicate academic publishing without adding real value. The peer-review system is demanding yet constructive, guided by genuine scientific dialogue rather than hierarchical or authoritarian attitudes. Reviewers act as collaborators in improving the manuscript, not as gatekeepers imposing arbitrary standards. This journal offers a rare balance: high methodological standards combined with a respectful, transparent, and supportive editorial approach. In an era where publishing can feel more burdensome than research itself, this platform restores the original purpose of peer review — to refine ideas, not to obstruct them Prof. Perlat Kapisyzi, FCCP PULMONOLOGIST AND THORACIC IMAGING.
Dear Reader: We have published several articles in the Auctores Publishing, LLC, journal, Clinical Medical Reviews and Reports in recent years (CMRR). This is an ‘open access’ journal and the following are our observations. From the initial invitation to submit an article, to the final edits of galley proofs, we have found CMRR personnel to be professional, responsive, rapid and thorough. This entire process begins with Catherine Mitchell, Editorial Coordinator. She is simply outstanding, and, I believe, unparalleled in her capacity. I cannot imagine a more responsive and dedicated Editorial Coordinator. As I read the dates and timing of her correspondence with us, it seems that she never sleeps. I hope Auctores Publishing, LLC, appreciates her efforts as much as these authors do. Thank you to Auctores Publishing, LLC, to the Editorial Staff/Board, and to Catherine Mitchell from a grateful author(s).
