Analytical Techniques for Obtaining Volatile oils (Odorants and Flavorings)

Review Article | DOI: https://doi.org/10.31579/2637-8914/304

Analytical Techniques for Obtaining Volatile oils (Odorants and Flavorings)

  • Bianca- Valentina Recsky
  • Ioana Grozea
  • Monica Butnariu *

University of Life Sciences "King Mihai I" from Timisoara, 300645, Calea Aradului 119, Timis, Romania.

*Corresponding Author: Monica Butnariu, Chemistry & Biochemistry Discipline, University of Life Sciences

Citation: Bianca- Valentina Recsky, Ioana Grozea, Monica Butnariu, (2025), Analytical Techniques for Obtaining Volatile oils (Odorants and Flavorings), J. Nutrition and Food Processing, 8(4); DOI:10.31579/2637-8914/304

Copyright: © 2025, Monica Butnariu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: 10 March 2025 | Accepted: 24 March 2025 | Published: 27 March 2025

Keywords: volatile oils; complex mixtures; entrainable liquids; volatilize

Abstract

Volatile oils are complex mixtures of aliphatic, aromatic and hydroaromatic hydrocarbons, aldehydes, alcohols, esters and other constituents in which compounds from the terpenoid class predominate. They have a characteristic, aromatic and pleasant smell. They are liquids that can be carried by water vapor. The name volatile oils is appropriate because it expresses the characteristic property of these compounds: high vapor pressure and the fact that they volatilize at ordinary temperature. Volatile oils are relatively widespread in the plant kingdom, some families being very rich in such substances, both in number of species and in quantity. Several variants and systems are used to obtain volatile oils, which are based on their property of being carried by water vapor, as a result of the high vapor pressure that characterizes them.

Introduction

The process used to obtain these natural substances depends on the way they are contained in the plant raw material, their chemical nature and their properties. Most of the production processes are based on two physical properties of odorants and flavorings, namely: the property of being entrained with water vapor and their solubility in certain solvents (hydrocarbons, alcohols, halogenated organic compounds, fats, carbon dioxide, etc.). The main processes applied in practice are the following: entrained with water vapor, extraction with animal fats, extraction with organic solvents, extraction with liquefied gases, extraction by pressing, adsorption on an adsorbent material. Among these, the most representative families are: Pinaceae, Labiatae, Umbelliferae, Myrtaceae, Lauraceae, Rutaceae, Cariophylaceae, Compositae, Zingiberaceae, etc., which are considered aromatic plants. The name aromatic plants is assigned to those species that contain at least 0.1-0.2% volatile oil and have a sufficiently perceptible odor. In addition to these, there are other species that, although they do not have a characteristic odor, still contain terpene substances that are part of the composition of vegetable oils (Monteiro, et al, 2010).

There is almost no organ in the composition of aromatic plants, in which, in various species, there are no larger or smaller amounts of volatile oils. Such are valerian roots, ginger rhizomes, lobelia leaves, rose flowers, citrus fruits, almond seeds (Batish, et al, 2006).

A. Chemical methods

a) Distillation with water (hydrodistillation)

In this process, the raw material is brought together with water into the distillation flask, the bottom of the container is heated directly at the heat source, and the mixture of oil vapor and water is collected by condensation in a cooling system. The distillate is then passed into a collecting vessel.

The method, although simple and easy to perform, nevertheless presents a number of disadvantages:

  • on the inner wall of the flask adhere the plant parts that can be carbonized, degrading the active principles;
  • in water a series of substances can be solubilized that distill together with the volatile oils, as a result of the formation of azeotropic systems;
  • due to the overheating of the walls of the distillation container, a series of components of the volatile oil, consisting of esters, can hydrolyze (linoleic acetate), cyclizations and oxidations occur (limonene, citral).
  • the process is discontinuous, increasing the specific consumptions (Ebrahimi, et al, 2022).

b) Water vapor entrainment

The raw material is brought into a metal sieve basket, which may or may not be previously closed in sackcloth, so that it does not come into contact with the water in the lower part of the blaza. The water vapor formed penetrates the vegetable material, entraining at the same time the volatile oils. This eliminates a number of disadvantages of the first process.

However, this method has some disadvantages:

  • water vapor can condense on the plant and thus extract other components that reduce the quality of the volatile oil;
  • water must be replenished from time to time, the process being discontinuous as a result of replacing batches of raw material after they have been used.

To overcome these disadvantages, a separate steam generator can be used. The method has the advantage that a single steam generator can serve a battery of stills. This generator can be placed in a different building than the one where the distillation flasks are installed. An advantage of this system is that the extraction is no longer interrupted to replenish the distilled water. In addition, when the contents of a distillation vessel have been exhausted, it can be replaced without interrupting the other stills (Ge, et al, 2000).

c) Extraction with volatile solvents

This process is applied to obtain fine volatile oils, for those with labile components or existing in minute quantities in the raw material. The method has the following advantages: extraction takes place at low temperatures; the possibility of resenification is avoided; the fine scent is protected; a series of resins are extracted, not evaporable with water vapor, but fixative of the scent.

The disadvantages of this procedure are: high cost; complexity of the installation; toxicity and explosion hazard of solvents.The most commonly used solvent is petroleum ether. This meets most of the required conditions, but preliminary purification is necessary to remove the upper fractions with an unpleasant odor. Extraction gasoline has a high boiling point and contains less volatile fractions. It is used mainly for obtaining fatty oils and to a lesser extent for volatile ones. Benzene is sufficiently pure, but dissolves too many ballast substances, and when mixed with air it is detonating. Ethyl ether is quite often used, but it has a high cost price, dissolves ballast substances and is flammable. Liquefied gases, such as carbon dioxide or liquid nitrogen, are considered ideal solvents, because they leave no residue on evaporation, have no odor, are not explosive and have a high dissolving capacity. Although it is flammable, the use of liquefied butane as an extraction solvent has recently been gaining more and more ground. Purification of the crude volatile oil is achieved by trituration with alcohol, followed by filtration of the insoluble parts. The filtrate is cooled to a temperature of -10...-15 °C, when most of the ballast leaves the system. It is filtered cold. The alcoholic solution is concentrated under vacuum, at a temperature as low as possible, after which, by entrainment with water vapor, the volatile oil is obtained (Piras, et al, 2022).

d) Extraction with non-volatile solvents

It can be done by several methods, namely:

• Maceration

The solvents used are usually mixtures of various fats, depending on the extraction process and the nature of the material treated. Thus, the following are used: animal fats (pork, tallow, or their mixture called corpus), vegetable fats (olive, almond, apricot or peach oil), synthetic substances (benzyl benzoate). The fats used as non-volatile solvents must be very pure, not containing residues of protein substances or water. In the extraction process, the flowers packed in rare cloth bags are brought in several batches into tinned iron boilers, which contain the corpus melted at a maximum of 60-70°C. A time is left for maceration, after which the bags are removed from the fat and a new batch of fresh flowers is brought. The operation is repeated approximately 25 times. The product obtained with the help of animal fats is called pomade, and the one with vegetable oils is called antique oil. The separation of volatile oils from the fat mixture is achieved by draining the melted fat from the maceration container and drying it on anhydrous sodium sulfate. Next, after filtration, it is cooled and stirred for a long time with alcohol. It is then cooled to freezing temperature, when the alcohol that has absorbed the volatile oil is separated from the fat. The product obtained is called flower extract. By distilling the alcohol under reduced pressure, the specific flower oil is obtained. The recovered alcohol is used for a new extraction (Hafez Ghoran, et al, 2021).

• Enfleurage

It is an expensive process through which, however, volatile oils of the best quality are obtained. In this case, the solvent, made up only of solid animal fats, retains only the most volatile fractions that are released from the flowers. The system in which the extraction of the volatile oil is carried out is made up of the so-called chassis, made up of a wooden frame, 3-5 cm high, which has a thick glass bottom. On the glass bottom of the chassis, a layer of solvent is spread about. 1 cm thick over which petals or whole fresh flowers are laid, as much as they can fit.

The maceration of the flowers takes place in the chassis placed in cool rooms where they are kept for 12-72 hours. During maceration, the volatile fractions contained in the flower petals saturate the space available to them. At the same time, some of these volatile compounds are absorbed by the solid fat, which causes the saturation of the space to decrease. In this way, a new amount of volatile oil is released from the flowers, and the process continues until an equilibrium is established that corresponds to a certain depletion of the flowers in volatile components. As the depletion progresses, batches of flowers are replaced with fresh material, the operation being repeated 30-50 times, to ensure saturation of the corpus with volatile oil.

Sometimes, solid absorbents can be used instead of the corpus. It is recommended that the flowers be harvested before they are pollinated. After pollination, biochemical processes take place in the plant tissues that can change the composition of the volatile oil (Paibon, et al, 2011).

• Solid solvent extraction

In this case, the phenomenon of sorption is used. Two distinct processes can occur: capillary condensation, when volatile oil vapors agglomerate and liquefy in the pores of the absorbent, and chemisorption, when a chemical reaction takes place between the absorbed and the absorbent. Obtaining the volatile oil or desorption is done by extraction with alcohol. The following absorbents are used: activated carbon or silica gel (Won, et al, 2009). 

B. Mechanical methods

These methods are particularly applicable to the extraction of volatile oils contained in the pericarp of citrus fruits. Several variants of this method are known:

a) By squeezing

The fresh fruit, cut into two or three parts, is cleaned of the core. The remaining peel is squeezed into a collecting vessel.Sometimes, before squeezing, the peels are kept in piles for one or two days, when a slight fermentation takes place, after which they are soaked for a few hours in water and then squeezed. Along with the volatile oil, part of the cell juice is also squeezed. After collection, the aqueous liquid is allowed to separate and then the oil is decanted (Perez-Cacho, & Rouseff, 2008).

b) By shaving

The surface of the fruit is scraped with a needle grater or one or two fruits are rotated in a spiked funnel or in a cylindrical vessel whose bottom is covered with short spikes arranged in concentric circles. The spikes must be sized so as to perforate only the oil glands, since, penetrating too deeply into the pericarp of the fruit, they increase the amount of cellular fluid, which will decrease the quality of the volatile oil (Uter, et al, 2007).

c) By pressing

Hand presses, screw presses and hydraulic presses are used for this purpose. The latter method gives the highest yields, but the best quality is provided by the manual method (Li, et al, 2021).

C. Fermentative methods

It is applied to obtain unformed volatile oils, which are found in plants in the form of glycosides.

First, the glycosides are cleaved, by fermentation, with the help of enzymes that are usually found in the same plant. Some products are processed immediately, others are stored from a few hours to a few months, during which the enzymatic cleavage of the glycosides takes place.

In order to carry out this method, the plant material is crushed and mixed with water.

A small fraction is set aside, and the rest is heated to 70-80°C to dissolve the glycosides, when, at the same time, the enzymes are inactivated. To cause fermentation, the unheated part (which contains the enzymes in the active state) is added, then left for a while for fermentation.

The volatile oil, set free, by this enzymatic pathway is carried away by distillation of the mixture. The volatile oil, obtained by the described processes, is collected in collectors, from where it is sent to the settling vessels, where it remains for a variable time depending on the characteristics of the oil and the dispersion of impurities. Usually, settling batteries are used (Zhang, et al, 2019).

After settling, the oil is dehydrated with anhydrous sodium sulfate, sometimes with calcined sodium chloride. The dehydrated oil is filtered through a pleated paper filter.

From the sodium sulfate used for dehydration, as well as from the used filters, the oil is recovered by entraining them with water vapor or by introducing them into the entraining flasks, together with the plant. All purification and recovery operations are carried out in the laboratory, in glass apparatus of reduced capacity and protected from the harmful action of some physico-chemical agents (oxidants, heavy metals, etc.). To obtain high-quality volatile oils, by removing the fractions that disqualify the product, fractional distillation is used. In this way, those components that give an unpleasant odor are removed, such as amines or furfural and some irritating compounds, mainly made up of aldehydes. Volatile oils are liquid substances, very volatile, with an aromatic and pleasant odor. Due to their high vapor pressure, they form azeotropic mixtures with water vapor and distill at much lower temperatures.They boil at temperatures between 150-300°C and are lighter than water. The exceptions are cinnamon, bitter almond and clove oils, which are heavier than water (Zhang, et al, 2018).

They are substances with optical activity, with characteristic refractive indices, with low viscosity, some being very fluid. They are soluble in organic solvents, insoluble in water, soluble in 60% chloral hydrate solution, themselves being very good organic solvents.

Volatile oils are flammable substances. Their odor can sometimes be irritating, although aromatic, and their taste is burning. 

Depending on its components, each volatile oil has specific chemical properties.

Analytical control of volatile oils

In addition to the characteristic odor, the various volatile oils can be identified by the major component, which can be recognized by characteristic identification reactions.

Thin-layer chromatography has been widely used recently for the rapid identification and overall assessment of the quality of volatile oils (Kotra, Satyabanta, & Goswami, 2019).

For a more in-depth analysis, the volatile oil is subjected to a fractionation (figure 1), which obtains the neutral components on the one hand, and the acidic components on the other.

Figure 1: Fractionation of volatile oil

The fractions thus obtained are subjected to chromatographic analysis on silica gel plates. As developing solvents, mixtures based on hexane-ethyl ether, hexane-ethyl acetate, benzene-toluene-ethyl acetate, benzene-chloroform, benzene-acetic acid, etc. are used. Strong reagents are used for development, as follows: 

  • concentrated sulfuric acid, when different colorations are obtained, according to the chemical nature of the fractions in the separate spots;
  • mixture of vanillin and concentrated sulfuric acid, when colorations and fluorescences are obtained, especially with the phenolic fractions;
  • anisaldehyde;
  • 4-amino-antipyrine and potassium ferricyanide, which give a red coloration; after subsequent spraying with ammonia, only the spots corresponding to the phenolic combinations remain colored red;
  • dinitro-phenylhydrazine, which provides yellow colorations with the carbonyl combinations (aldehydes and ketones).

To determine the quality of a volatile oil, a series of physical and chemical tests are performed, which highlight specific characteristics. To perform a correct analysis, the volatile oil sample must be clear (freshly filtered) and free of water (dried on anhydrous sodium sulfate) (Zhang, et al, 2021).

Among the physical characteristics necessary for the characterization of a volatile oil, the following should be mentioned: olfactory examination, density at 20°C (d2020), specific rotation angle (nD20), refractive index at 20°C (nD20), melting point, freezing point, dropping point, solubility, clarity, iodine color, evaporation residue, ultraviolet and visible spectrograms, gas chromatogram, thin layer chromatogram (Romanenko, Domrachev, & Tkachev, 2022).

The most important chemical characteristics that must be performed, partially or entirely, to characterize a volatile oil are: acidity index, esterification index, saponification index, acetyl index, ester index, carbonyl index, alcohol content (%), aldehyde and ketone content (%), phenol content (%), solubility in 5% sodium hydroxide solution, determination of sulfur derivatives, determination of nitrogen derivatives, special determinations.

Conclusions and recommendations

In general, volatile oils stored in the light turn brown as a result of photochemical oxidation reactions. Their oxidizability is explained by the existence of double bonds as well as oxydryl and carbonyl functions grafted onto the chain or the basic nucleus. As a result, some of the oil components easily pass into macromolecular condensation compounds.

With concentrated sulfuric acid they give intense colors.

Volatile oils are used in various medications, in the form of crude plant products or extracted oils, as well as as semi-synthetic material, for obtaining other medicinal substances (for example: obtaining synthetic camphor, hydrated terpine, etc.).

In pharmacy, volatile oils are used as flavorings and taste correctors.

They are widely used in cosmetics and perfumery, when used in the form of extracts, hydrolates, alcoholates, tinctures, concretes, resinoids and oleoresins, essences, perfumes, etc. From these, beauty and maintenance cosmetics are prepared in the form of creams, emulsions, lotions, gels, oils, masks, make-up removers, soaps, bath and shower preparations, deodorants and antiperspirants, moisturizers, sunscreens, as well as numerous shaving preparations. Special attention is paid to preparations for dental and hair care, as well as products with a preventive and curative role in dermopharmacy.

Also, volatile oils are successfully used in the maintenance of public premises (fir oil), in microscopy (cedar oil), in the lens manufacturing technique (Canada balsam), in chromatographic techniques, in the varnish and paint industry, as insecticides and as flotation oil (pine oil) in the mining industry.

References

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Dr Maria Regina Penchyna Nieto

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.

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Dr Marcelo Flavio Gomes Jardim Filho

Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”

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Zsuzsanna Bene

Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner

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Dr Susan Weiner

My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.

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Lin-Show Chin

My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.

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Sonila Qirko

My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.

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Luiz Sellmann

I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.

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Zhao Jia

Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."

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Thomas Urban

I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.

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Cristina Berriozabal

To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.

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Dr Tewodros Kassahun Tarekegn

"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".

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Dr Shweta Tiwari

I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.

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Dr Farooq Wandroo

Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.

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Dr Anyuta Ivanova

We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.

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Dr David Vinyes

My article, titled 'No Way Out of the Smartphone Epidemic Without Considering the Insights of Brain Research,' has been republished in the International Journal of Clinical Case Reports and Reviews. The review process was seamless and professional, with the editors being both friendly and supportive. I am deeply grateful for their efforts.

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Gertraud Teuchert-Noodt

To Dear Erin Aust – Editorial Coordinator of Journal of General Medicine and Clinical Practice! I declare that I am absolutely satisfied with your work carried out with great competence in following the manuscript during the various stages from its receipt, during the revision process to the final acceptance for publication. Thank Prof. Elvira Farina

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Dr Elvira Farina